OS backup ? you can run an online progress backup ( probkup util ) while
users are connected and working, we do it all the time.
Depending on the version you're working on, you can use probkup with
private read-only buffers ( -Bp ), so the util wouldn't monopolize the buffer
pool and minimizes the impact.
There are far more options and features using the Progress purposely built
backup/restore/roll-forward etc. features then an external OS backup e.g.
incremental backup, after-imaging, roll-forward to a certain time etc.
But you can easily disconnect all users before the backup runs.
Even if there's no activity while the backup is running, it's typically not
recommended to routinely stop the database or disconnect all users and/or
servers.